wt probe Search Results


wt allele (fam probe  (Eurofins)
90
Eurofins wt allele (fam probe
Wt Allele (Fam Probe, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wt allele (fam probe/product/Eurofins
Average 90 stars, based on 1 article reviews
wt allele (fam probe - by Bioz Stars, 2026-05
90/100 stars
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biotin-labeled mir-141-3p probe wt-bio-mir-141-3p  (CloudSeq Biotech Inc)
90
CloudSeq Biotech Inc biotin-labeled mir-141-3p probe wt-bio-mir-141-3p
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Biotin Labeled Mir 141 3p Probe Wt Bio Mir 141 3p, supplied by CloudSeq Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin-labeled mir-141-3p probe wt-bio-mir-141-3p/product/CloudSeq Biotech Inc
Average 90 stars, based on 1 article reviews
biotin-labeled mir-141-3p probe wt-bio-mir-141-3p - by Bioz Stars, 2026-05
90/100 stars
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probe for kras wt  (ACGT Inc)
90
ACGT Inc probe for kras wt
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Probe For Kras Wt, supplied by ACGT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/probe for kras wt/product/ACGT Inc
Average 90 stars, based on 1 article reviews
probe for kras wt - by Bioz Stars, 2026-05
90/100 stars
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probes na-wt and na-tamiflur  (Biosearch Technologies Inc)
90
Biosearch Technologies Inc probes na-wt and na-tamiflur
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Probes Na Wt And Na Tamiflur, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/probes na-wt and na-tamiflur/product/Biosearch Technologies Inc
Average 90 stars, based on 1 article reviews
probes na-wt and na-tamiflur - by Bioz Stars, 2026-05
90/100 stars
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human whole transcriptome (wt) probes  (10X Genomics)
90
10X Genomics human whole transcriptome (wt) probes
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Human Whole Transcriptome (Wt) Probes, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human whole transcriptome (wt) probes/product/10X Genomics
Average 90 stars, based on 1 article reviews
human whole transcriptome (wt) probes - by Bioz Stars, 2026-05
90/100 stars
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spotting probe wt  (Metabion International AG)
90
Metabion International AG spotting probe wt
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Spotting Probe Wt, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spotting probe wt/product/Metabion International AG
Average 90 stars, based on 1 article reviews
spotting probe wt - by Bioz Stars, 2026-05
90/100 stars
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nonfluorescent probe wt  (Assay Designs Inc)
90
Assay Designs Inc nonfluorescent probe wt
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Nonfluorescent Probe Wt, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nonfluorescent probe wt/product/Assay Designs Inc
Average 90 stars, based on 1 article reviews
nonfluorescent probe wt - by Bioz Stars, 2026-05
90/100 stars
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wt – ph inolab probe  (Xylem Analytics)
90
Xylem Analytics wt – ph inolab probe
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Wt – Ph Inolab Probe, supplied by Xylem Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wt – ph inolab probe/product/Xylem Analytics
Average 90 stars, based on 1 article reviews
wt – ph inolab probe - by Bioz Stars, 2026-05
90/100 stars
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human wt probes v2, lhs  (10X Genomics)
90
10X Genomics human wt probes v2, lhs
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Human Wt Probes V2, Lhs, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human wt probes v2, lhs/product/10X Genomics
Average 90 stars, based on 1 article reviews
human wt probes v2, lhs - by Bioz Stars, 2026-05
90/100 stars
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sars-cov-2 s proteins  (10X Genomics)
90
10X Genomics sars-cov-2 s proteins
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Sars Cov 2 S Proteins, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sars-cov-2 s proteins/product/10X Genomics
Average 90 stars, based on 1 article reviews
sars-cov-2 s proteins - by Bioz Stars, 2026-05
90/100 stars
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conductivity probe amprobe wt-60  (Amprobe GmbH)
90
Amprobe GmbH conductivity probe amprobe wt-60
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Conductivity Probe Amprobe Wt 60, supplied by Amprobe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conductivity probe amprobe wt-60/product/Amprobe GmbH
Average 90 stars, based on 1 article reviews
conductivity probe amprobe wt-60 - by Bioz Stars, 2026-05
90/100 stars
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ruthenium-labeled wt (5′-ccttatgagaaatcaaagtc 3′) detection probes  (Organon Teknika Corporation LLC)
90
Organon Teknika Corporation LLC ruthenium-labeled wt (5′-ccttatgagaaatcaaagtc 3′) detection probes
<t>MiR-141-3p</t> is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.
Ruthenium Labeled Wt (5′ Ccttatgagaaatcaaagtc 3′) Detection Probes, supplied by Organon Teknika Corporation LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ruthenium-labeled wt (5′-ccttatgagaaatcaaagtc 3′) detection probes/product/Organon Teknika Corporation LLC
Average 90 stars, based on 1 article reviews
ruthenium-labeled wt (5′-ccttatgagaaatcaaagtc 3′) detection probes - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


MiR-141-3p is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.

Journal: Drug Delivery

Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis

doi: 10.1080/10717544.2022.2030428

Figure Lengend Snippet: MiR-141-3p is a target of circDIDO1. (A) qRT-PCR analysis for the expression of miR-141-3p, miR-128-3p, miR-188-5p, miR-942-5p, and miR-217 in LX2 cells transfected with circDIDO1 or vector. (B) RIP experiment was performed with Ago2 antibody and negative control IgG, followed by qRT-PCR to detect the expression of miR-141-3p and circDIDO1. (C) RNA pull-down assay was performed using the miR-141-3p-specific probe and control probe, and the enrichment of circDIDO1 was detected by qRT-PCR. (D) The starBase database showing the binding site of miR-141-3p on circDIDO1, and the mutated sequence of circDIDO1 in the binding site was established. (E) qRT-PCR analysis for miR-141-3p expression level in LX2 cells after miR-141-3p mimic or NC mimic transfection. (F) Dual-luciferase reporter assay for the luciferase activity of wild and mutated circDIDO1 reporter after miR-141-3p overexpression in LX2 cells. * p < .05, ** p < .01, *** p < .001.

Article Snippet: Biotin-labeled miR-141-3p probe (wt-bio-miR-141-3p and mut-miR-141-3p) and control probe (bio-NC) were synthesized by CloudSeq Biotech Inc. (Shanghai, China).

Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, Negative Control, Pull Down Assay, Binding Assay, Sequencing, Luciferase, Reporter Assay, Activity Assay, Over Expression

MiR-141-3p knockdown suppresses the activation of HSCs by PTEN/AKT pathway. (A–G) LX2 cells were transfected with miR-141-3p inhibitor or NC inhibitor. (A) qRT-PCR analysis for the expression level of miR-141-3p in LX2 cells after transfection. (B, C) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (D, E) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (F, G) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.

Journal: Drug Delivery

Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis

doi: 10.1080/10717544.2022.2030428

Figure Lengend Snippet: MiR-141-3p knockdown suppresses the activation of HSCs by PTEN/AKT pathway. (A–G) LX2 cells were transfected with miR-141-3p inhibitor or NC inhibitor. (A) qRT-PCR analysis for the expression level of miR-141-3p in LX2 cells after transfection. (B, C) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (D, E) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (F, G) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.

Article Snippet: Biotin-labeled miR-141-3p probe (wt-bio-miR-141-3p and mut-miR-141-3p) and control probe (bio-NC) were synthesized by CloudSeq Biotech Inc. (Shanghai, China).

Techniques: Activation Assay, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Western Blot

CircDIDO1 suppresses the activation of HSCs by miR-141-3p/PTEN/AKT pathway. (A–F) LX2 cells were co-transfected with circDIDO1 and miR-141-3p mimic. (A, B) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (C, D) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (E, F) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.

Journal: Drug Delivery

Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis

doi: 10.1080/10717544.2022.2030428

Figure Lengend Snippet: CircDIDO1 suppresses the activation of HSCs by miR-141-3p/PTEN/AKT pathway. (A–F) LX2 cells were co-transfected with circDIDO1 and miR-141-3p mimic. (A, B) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (C, D) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (E, F) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.

Article Snippet: Biotin-labeled miR-141-3p probe (wt-bio-miR-141-3p and mut-miR-141-3p) and control probe (bio-NC) were synthesized by CloudSeq Biotech Inc. (Shanghai, China).

Techniques: Activation Assay, Transfection, CCK-8 Assay, Flow Cytometry, Western Blot

MSC-exosome-mediated transfer of circDIDO1 suppresses the activation of HSCs by PTEN/AKT pathway. (A–H) LX2 cells were co-cultured with PBS, MSC-circDIDO1 Exo or MSC-vector Exo. (A) qRT-PCR analysis of circDIDO1 and miR-141-3p expression level in LX2 cells after incubation. (C, D) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (E, F) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (G, H) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.

Journal: Drug Delivery

Article Title: Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosis

doi: 10.1080/10717544.2022.2030428

Figure Lengend Snippet: MSC-exosome-mediated transfer of circDIDO1 suppresses the activation of HSCs by PTEN/AKT pathway. (A–H) LX2 cells were co-cultured with PBS, MSC-circDIDO1 Exo or MSC-vector Exo. (A) qRT-PCR analysis of circDIDO1 and miR-141-3p expression level in LX2 cells after incubation. (C, D) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (E, F) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (G, H) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. * p < .05, ** p < .01, and *** p < .001.

Article Snippet: Biotin-labeled miR-141-3p probe (wt-bio-miR-141-3p and mut-miR-141-3p) and control probe (bio-NC) were synthesized by CloudSeq Biotech Inc. (Shanghai, China).

Techniques: Activation Assay, Cell Culture, Plasmid Preparation, Quantitative RT-PCR, Expressing, Incubation, CCK-8 Assay, Flow Cytometry, Western Blot